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mouse spleen magcellect mouse nk cell isolation kit  (R&D Systems)


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    R&D Systems mouse spleen magcellect mouse nk cell isolation kit
    FIGURE 3. Rescue of defective degranulation of Munc13-4 KO <t>NK</t> <t>cells</t> with lentivirus expressing WT Munc13-4. Degranulation assays of Munc13-4 KO pri- mary NK cells that were transduced with lentivirus expressing EmGFP or WT Munc13-4–EmGFP. Degran- ulation was triggered with 2.5 mM ionomycin + 0.1 mM PMA for 6 h, and surface expression of CD107a was measured by FACS analysis. (A) Contour plots of FACS analysis illustrating induction of CD107a surface ex- pression in NK1.1+ primary NK cells that were trans- duced with EmGFP or WT Munc13-4 in unstimulated (top) and stimulated (bottom) condition. (B) Overlay of CD107a histograms in unstimulated (black) and stimu- lated (gray) NK cells. (C) A bar graph showing CD107a degranulation from EmGFP-rescued (white) and WT Munc13-4–rescued (black) KO NK cells. Error bars in- dicate SEM (n = 4).
    Mouse Spleen Magcellect Mouse Nk Cell Isolation Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse spleen magcellect mouse nk cell isolation kit/product/R&D Systems
    Average 92 stars, based on 8 article reviews
    mouse spleen magcellect mouse nk cell isolation kit - by Bioz Stars, 2026-03
    92/100 stars

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    1) Product Images from "C2 Domains of Munc13-4 Are Crucial for Ca 2+ -Dependent Degranulation and Cytotoxicity in NK Cells."

    Article Title: C2 Domains of Munc13-4 Are Crucial for Ca 2+ -Dependent Degranulation and Cytotoxicity in NK Cells.

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    doi: 10.4049/jimmunol.1800426

    FIGURE 3. Rescue of defective degranulation of Munc13-4 KO NK cells with lentivirus expressing WT Munc13-4. Degranulation assays of Munc13-4 KO pri- mary NK cells that were transduced with lentivirus expressing EmGFP or WT Munc13-4–EmGFP. Degran- ulation was triggered with 2.5 mM ionomycin + 0.1 mM PMA for 6 h, and surface expression of CD107a was measured by FACS analysis. (A) Contour plots of FACS analysis illustrating induction of CD107a surface ex- pression in NK1.1+ primary NK cells that were trans- duced with EmGFP or WT Munc13-4 in unstimulated (top) and stimulated (bottom) condition. (B) Overlay of CD107a histograms in unstimulated (black) and stimu- lated (gray) NK cells. (C) A bar graph showing CD107a degranulation from EmGFP-rescued (white) and WT Munc13-4–rescued (black) KO NK cells. Error bars in- dicate SEM (n = 4).
    Figure Legend Snippet: FIGURE 3. Rescue of defective degranulation of Munc13-4 KO NK cells with lentivirus expressing WT Munc13-4. Degranulation assays of Munc13-4 KO pri- mary NK cells that were transduced with lentivirus expressing EmGFP or WT Munc13-4–EmGFP. Degran- ulation was triggered with 2.5 mM ionomycin + 0.1 mM PMA for 6 h, and surface expression of CD107a was measured by FACS analysis. (A) Contour plots of FACS analysis illustrating induction of CD107a surface ex- pression in NK1.1+ primary NK cells that were trans- duced with EmGFP or WT Munc13-4 in unstimulated (top) and stimulated (bottom) condition. (B) Overlay of CD107a histograms in unstimulated (black) and stimu- lated (gray) NK cells. (C) A bar graph showing CD107a degranulation from EmGFP-rescued (white) and WT Munc13-4–rescued (black) KO NK cells. Error bars in- dicate SEM (n = 4).

    Techniques Used: Expressing, Transduction

    FIGURE 4. Double and quadruple muta- tions in the C2 domains of Munc13-4 alters Ca2+ sensitivity of degranulation from NK cells. Degranulation assays of Munc13-4 KO primary NK cells that were transduced with lentivirus expressing EmGFP alone, WT, C2 domain double, or quadruple mu- tant Munc13-4–EmGFP. Degranulation was triggered with 2.5 mM ionomycin + 0.1 mM PMA for 6 h and surface expression of CD107a was measured by FACS analysis. (A) Contour plots of FACS analysis illus- trating induction of CD107a surface ex- pression in NK1.1+ primary NK cells in 2.2 mM (left) and 10 mM (right) extracel- lular Ca2+. (B) Overlay of CD107a histo- grams in 2.2 mM (black) and 10 mM (gray) NK cells. (C) A bar graph showing CD107a degranulation from rescued NK cells in 2.2 mM (white) and 10 mM (black). Error bars indicate SEM (n 5 4). *p , 0.05.
    Figure Legend Snippet: FIGURE 4. Double and quadruple muta- tions in the C2 domains of Munc13-4 alters Ca2+ sensitivity of degranulation from NK cells. Degranulation assays of Munc13-4 KO primary NK cells that were transduced with lentivirus expressing EmGFP alone, WT, C2 domain double, or quadruple mu- tant Munc13-4–EmGFP. Degranulation was triggered with 2.5 mM ionomycin + 0.1 mM PMA for 6 h and surface expression of CD107a was measured by FACS analysis. (A) Contour plots of FACS analysis illus- trating induction of CD107a surface ex- pression in NK1.1+ primary NK cells in 2.2 mM (left) and 10 mM (right) extracel- lular Ca2+. (B) Overlay of CD107a histo- grams in 2.2 mM (black) and 10 mM (gray) NK cells. (C) A bar graph showing CD107a degranulation from rescued NK cells in 2.2 mM (white) and 10 mM (black). Error bars indicate SEM (n 5 4). *p , 0.05.

    Techniques Used: Transduction, Expressing

    FIGURE 5. Single-point mutations in the C2 domains of Munc13-4 alter Ca2+ sensi- tivity of degranulation from NK cells. De- granulation assays of Munc13-4 KO primary NK cells that were transduced with lentivirus expressing EmGFP alone, WT, or C2 domain single-mutant Munc13-4 EmGFP. Degranula- tion was triggered with 2.5 mM ionomycin + 0.1 mM PMA for 6 h and surface expression of CD107a was measured by FACS analysis. (A) Contour plots of FACS analysis illustrat- ing induction of CD107a surface expression in NK1.1+ primary NK cells in 2.2 mM (left) and 10 mM (right) extracellular Ca2+. (B) Overlay of CD107a histograms in 2.2 mM (black) and 10 mM (gray) NK cells. (C) A bar graph showing CD107a degranulation from rescued NK cells in 2.2 mM (white) and 10 mM (black). Error bars indicate SEM (n 5 4). *p , 0.05.
    Figure Legend Snippet: FIGURE 5. Single-point mutations in the C2 domains of Munc13-4 alter Ca2+ sensi- tivity of degranulation from NK cells. De- granulation assays of Munc13-4 KO primary NK cells that were transduced with lentivirus expressing EmGFP alone, WT, or C2 domain single-mutant Munc13-4 EmGFP. Degranula- tion was triggered with 2.5 mM ionomycin + 0.1 mM PMA for 6 h and surface expression of CD107a was measured by FACS analysis. (A) Contour plots of FACS analysis illustrat- ing induction of CD107a surface expression in NK1.1+ primary NK cells in 2.2 mM (left) and 10 mM (right) extracellular Ca2+. (B) Overlay of CD107a histograms in 2.2 mM (black) and 10 mM (gray) NK cells. (C) A bar graph showing CD107a degranulation from rescued NK cells in 2.2 mM (white) and 10 mM (black). Error bars indicate SEM (n 5 4). *p , 0.05.

    Techniques Used: Transduction, Expressing, Mutagenesis

    FIGURE 6. Mutations in C2 domains of Munc13-4 drastically reduces cytotoxicity of NK cells. (A) Control and Munc13-4 KO NK cells (effector cells) were incubated with Calcein Blue AM–loaded K562 cells (target cells) at indicated E:T ratios for 4 h, and the amount of Calcein Blue AM was measured. Percentage of lysis was calculated by dividing the amount of Calcein Blue AM by the total amounts (2% Triton X-100) 3 100%. Error bars indicate SEM (n = 12). (B) Cytotoxicity assay of Munc13-4 KO NK cells that are transduced with lentivirus expressing EmGFP, WT, or indicated C2 domain mutants. E:T ratios were 50:1 and 12.5:1. Error bars indicate SEM (n = 9).
    Figure Legend Snippet: FIGURE 6. Mutations in C2 domains of Munc13-4 drastically reduces cytotoxicity of NK cells. (A) Control and Munc13-4 KO NK cells (effector cells) were incubated with Calcein Blue AM–loaded K562 cells (target cells) at indicated E:T ratios for 4 h, and the amount of Calcein Blue AM was measured. Percentage of lysis was calculated by dividing the amount of Calcein Blue AM by the total amounts (2% Triton X-100) 3 100%. Error bars indicate SEM (n = 12). (B) Cytotoxicity assay of Munc13-4 KO NK cells that are transduced with lentivirus expressing EmGFP, WT, or indicated C2 domain mutants. E:T ratios were 50:1 and 12.5:1. Error bars indicate SEM (n = 9).

    Techniques Used: Control, Incubation, Lysis, Cytotoxicity Assay, Transduction, Expressing



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    mouse spleen magcellect mouse nk cell isolation kit  (R&D Systems)
    92
    R&D Systems mouse spleen magcellect mouse nk cell isolation kit
    FIGURE 3. Rescue of defective degranulation of Munc13-4 KO <t>NK</t> <t>cells</t> with lentivirus expressing WT Munc13-4. Degranulation assays of Munc13-4 KO pri- mary NK cells that were transduced with lentivirus expressing EmGFP or WT Munc13-4–EmGFP. Degran- ulation was triggered with 2.5 mM ionomycin + 0.1 mM PMA for 6 h, and surface expression of CD107a was measured by FACS analysis. (A) Contour plots of FACS analysis illustrating induction of CD107a surface ex- pression in NK1.1+ primary NK cells that were trans- duced with EmGFP or WT Munc13-4 in unstimulated (top) and stimulated (bottom) condition. (B) Overlay of CD107a histograms in unstimulated (black) and stimu- lated (gray) NK cells. (C) A bar graph showing CD107a degranulation from EmGFP-rescued (white) and WT Munc13-4–rescued (black) KO NK cells. Error bars in- dicate SEM (n = 4).
    Mouse Spleen Magcellect Mouse Nk Cell Isolation Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse spleen magcellect mouse nk cell isolation kit/product/R&D Systems
    Average 92 stars, based on 1 article reviews
    mouse spleen magcellect mouse nk cell isolation kit - by Bioz Stars, 2026-03
    92/100 stars
    		  Buy from Supplier

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    FIGURE 3. Rescue of defective degranulation of Munc13-4 KO NK cells with lentivirus expressing WT Munc13-4. Degranulation assays of Munc13-4 KO pri- mary NK cells that were transduced with lentivirus expressing EmGFP or WT Munc13-4–EmGFP. Degran- ulation was triggered with 2.5 mM ionomycin + 0.1 mM PMA for 6 h, and surface expression of CD107a was measured by FACS analysis. (A) Contour plots of FACS analysis illustrating induction of CD107a surface ex- pression in NK1.1+ primary NK cells that were trans- duced with EmGFP or WT Munc13-4 in unstimulated (top) and stimulated (bottom) condition. (B) Overlay of CD107a histograms in unstimulated (black) and stimu- lated (gray) NK cells. (C) A bar graph showing CD107a degranulation from EmGFP-rescued (white) and WT Munc13-4–rescued (black) KO NK cells. Error bars in- dicate SEM (n = 4).

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: C2 Domains of Munc13-4 Are Crucial for Ca 2+ -Dependent Degranulation and Cytotoxicity in NK Cells.

    doi: 10.4049/jimmunol.1800426

    Figure Lengend Snippet: FIGURE 3. Rescue of defective degranulation of Munc13-4 KO NK cells with lentivirus expressing WT Munc13-4. Degranulation assays of Munc13-4 KO pri- mary NK cells that were transduced with lentivirus expressing EmGFP or WT Munc13-4–EmGFP. Degran- ulation was triggered with 2.5 mM ionomycin + 0.1 mM PMA for 6 h, and surface expression of CD107a was measured by FACS analysis. (A) Contour plots of FACS analysis illustrating induction of CD107a surface ex- pression in NK1.1+ primary NK cells that were trans- duced with EmGFP or WT Munc13-4 in unstimulated (top) and stimulated (bottom) condition. (B) Overlay of CD107a histograms in unstimulated (black) and stimu- lated (gray) NK cells. (C) A bar graph showing CD107a degranulation from EmGFP-rescued (white) and WT Munc13-4–rescued (black) KO NK cells. Error bars in- dicate SEM (n = 4).

    Article Snippet: Isolation and culture of primary NK cells from mouse spleen MagCellect Mouse NK Cell Isolation Kit (catalog no. MAGM210) (R&D Systems, Minneapolis, MN) was used, following manufacturer’s instructions for isolation.

    Techniques: Expressing, Transduction

    FIGURE 4. Double and quadruple muta- tions in the C2 domains of Munc13-4 alters Ca2+ sensitivity of degranulation from NK cells. Degranulation assays of Munc13-4 KO primary NK cells that were transduced with lentivirus expressing EmGFP alone, WT, C2 domain double, or quadruple mu- tant Munc13-4–EmGFP. Degranulation was triggered with 2.5 mM ionomycin + 0.1 mM PMA for 6 h and surface expression of CD107a was measured by FACS analysis. (A) Contour plots of FACS analysis illus- trating induction of CD107a surface ex- pression in NK1.1+ primary NK cells in 2.2 mM (left) and 10 mM (right) extracel- lular Ca2+. (B) Overlay of CD107a histo- grams in 2.2 mM (black) and 10 mM (gray) NK cells. (C) A bar graph showing CD107a degranulation from rescued NK cells in 2.2 mM (white) and 10 mM (black). Error bars indicate SEM (n 5 4). *p , 0.05.

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: C2 Domains of Munc13-4 Are Crucial for Ca 2+ -Dependent Degranulation and Cytotoxicity in NK Cells.

    doi: 10.4049/jimmunol.1800426

    Figure Lengend Snippet: FIGURE 4. Double and quadruple muta- tions in the C2 domains of Munc13-4 alters Ca2+ sensitivity of degranulation from NK cells. Degranulation assays of Munc13-4 KO primary NK cells that were transduced with lentivirus expressing EmGFP alone, WT, C2 domain double, or quadruple mu- tant Munc13-4–EmGFP. Degranulation was triggered with 2.5 mM ionomycin + 0.1 mM PMA for 6 h and surface expression of CD107a was measured by FACS analysis. (A) Contour plots of FACS analysis illus- trating induction of CD107a surface ex- pression in NK1.1+ primary NK cells in 2.2 mM (left) and 10 mM (right) extracel- lular Ca2+. (B) Overlay of CD107a histo- grams in 2.2 mM (black) and 10 mM (gray) NK cells. (C) A bar graph showing CD107a degranulation from rescued NK cells in 2.2 mM (white) and 10 mM (black). Error bars indicate SEM (n 5 4). *p , 0.05.

    Article Snippet: Isolation and culture of primary NK cells from mouse spleen MagCellect Mouse NK Cell Isolation Kit (catalog no. MAGM210) (R&D Systems, Minneapolis, MN) was used, following manufacturer’s instructions for isolation.

    Techniques: Transduction, Expressing

    FIGURE 5. Single-point mutations in the C2 domains of Munc13-4 alter Ca2+ sensi- tivity of degranulation from NK cells. De- granulation assays of Munc13-4 KO primary NK cells that were transduced with lentivirus expressing EmGFP alone, WT, or C2 domain single-mutant Munc13-4 EmGFP. Degranula- tion was triggered with 2.5 mM ionomycin + 0.1 mM PMA for 6 h and surface expression of CD107a was measured by FACS analysis. (A) Contour plots of FACS analysis illustrat- ing induction of CD107a surface expression in NK1.1+ primary NK cells in 2.2 mM (left) and 10 mM (right) extracellular Ca2+. (B) Overlay of CD107a histograms in 2.2 mM (black) and 10 mM (gray) NK cells. (C) A bar graph showing CD107a degranulation from rescued NK cells in 2.2 mM (white) and 10 mM (black). Error bars indicate SEM (n 5 4). *p , 0.05.

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: C2 Domains of Munc13-4 Are Crucial for Ca 2+ -Dependent Degranulation and Cytotoxicity in NK Cells.

    doi: 10.4049/jimmunol.1800426

    Figure Lengend Snippet: FIGURE 5. Single-point mutations in the C2 domains of Munc13-4 alter Ca2+ sensi- tivity of degranulation from NK cells. De- granulation assays of Munc13-4 KO primary NK cells that were transduced with lentivirus expressing EmGFP alone, WT, or C2 domain single-mutant Munc13-4 EmGFP. Degranula- tion was triggered with 2.5 mM ionomycin + 0.1 mM PMA for 6 h and surface expression of CD107a was measured by FACS analysis. (A) Contour plots of FACS analysis illustrat- ing induction of CD107a surface expression in NK1.1+ primary NK cells in 2.2 mM (left) and 10 mM (right) extracellular Ca2+. (B) Overlay of CD107a histograms in 2.2 mM (black) and 10 mM (gray) NK cells. (C) A bar graph showing CD107a degranulation from rescued NK cells in 2.2 mM (white) and 10 mM (black). Error bars indicate SEM (n 5 4). *p , 0.05.

    Article Snippet: Isolation and culture of primary NK cells from mouse spleen MagCellect Mouse NK Cell Isolation Kit (catalog no. MAGM210) (R&D Systems, Minneapolis, MN) was used, following manufacturer’s instructions for isolation.

    Techniques: Transduction, Expressing, Mutagenesis

    FIGURE 6. Mutations in C2 domains of Munc13-4 drastically reduces cytotoxicity of NK cells. (A) Control and Munc13-4 KO NK cells (effector cells) were incubated with Calcein Blue AM–loaded K562 cells (target cells) at indicated E:T ratios for 4 h, and the amount of Calcein Blue AM was measured. Percentage of lysis was calculated by dividing the amount of Calcein Blue AM by the total amounts (2% Triton X-100) 3 100%. Error bars indicate SEM (n = 12). (B) Cytotoxicity assay of Munc13-4 KO NK cells that are transduced with lentivirus expressing EmGFP, WT, or indicated C2 domain mutants. E:T ratios were 50:1 and 12.5:1. Error bars indicate SEM (n = 9).

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: C2 Domains of Munc13-4 Are Crucial for Ca 2+ -Dependent Degranulation and Cytotoxicity in NK Cells.

    doi: 10.4049/jimmunol.1800426

    Figure Lengend Snippet: FIGURE 6. Mutations in C2 domains of Munc13-4 drastically reduces cytotoxicity of NK cells. (A) Control and Munc13-4 KO NK cells (effector cells) were incubated with Calcein Blue AM–loaded K562 cells (target cells) at indicated E:T ratios for 4 h, and the amount of Calcein Blue AM was measured. Percentage of lysis was calculated by dividing the amount of Calcein Blue AM by the total amounts (2% Triton X-100) 3 100%. Error bars indicate SEM (n = 12). (B) Cytotoxicity assay of Munc13-4 KO NK cells that are transduced with lentivirus expressing EmGFP, WT, or indicated C2 domain mutants. E:T ratios were 50:1 and 12.5:1. Error bars indicate SEM (n = 9).

    Article Snippet: Isolation and culture of primary NK cells from mouse spleen MagCellect Mouse NK Cell Isolation Kit (catalog no. MAGM210) (R&D Systems, Minneapolis, MN) was used, following manufacturer’s instructions for isolation.

    Techniques: Control, Incubation, Lysis, Cytotoxicity Assay, Transduction, Expressing